Human recombinant laminin-511

Culturing human and murine embryonic stem cells or induced pluripotent stem cells, in fully defined, consistent, and, thus, reproducible manner, is known to be difficult. For years, researchers have tried to find a solution to this central problem. BioLamina can now deliver the answer by providing human recombinant laminin for chemically defined stem cell culturing. The hES cells in this picture grow with retained pluripotency as homogenous monolayers for over 5 months or 20 passages on this laminin isoform. Laminin-511 (or laminin-10) is ideal for culturing pluripotent cells, including mouse embryonic stem cells. Laminin-511 substrate as a coating material for adherent cell cultures makes stem cell culturing easy and efficient. 

Gives you control over your cell culture conditions for maximal output.

Simulates the natural environment for your cultured cells.

Fully defined, animal protein free and can be made by GMP standard.

Increase repeatability and reliability

Pluripotent stem cells proliferate for months in culture keeping their phenotype on recombinant laminin-511.

Mouse and human ES and iPS cells grow on a completely defined matrix, without the need of using time-consuming and cumbersome feeder cells (Domogatskaya, 2008; Rodin, 2010).

Fewer replicates needed for reliable results on this laminin substrate, especially if used together with a defined culture medium.

Stem cells grow as monolayers on laminin-511 enabling equal contact to both the matrix and cell culture medium, which leads to a more homogenous cell population (Rodin, 2010). This is because ES cells have the highest affinity to laminin-511 of all known biological surfaces for ES cell culturing.

Biorelevance

The first extracellular protein in the embryo is laminin-511. It is expressed already in the inner cell mass of blastocysts and in the early embryo, which is why this laminin isoform works so well on pluripotent cells in vitro (Klaffky, 2001; Miner, 2004).

Mouse embryonic stem cells expand without the need of LIF, since the laminin-511 protein creates a biological niche keeping the ES cells in a pluripotent state for months (Domogatskaya, 2008).

Biorelevant conclusions from your research can be further used to develop cells for therapeutic purposes with GMP-grade laminins produced by BioLamina.

From bench to bedside

Laminin-511 is completely free of animal proteins. Together with a defined, xeno-free culture medium you can obtain a stem cell culture system devoid of contaminating animal proteins (Azarin and Palecek, 2010; Martin et al., 2005; Rodin et al., 2010).

Production according to GMP of clinical grade human recombinant laminins is feasible.

In the end, human recombinant laminins enable the production of GMP grade therapeutic cells, which facilitates entry to clinical trials.