Does your Biolaminin 332 product contain the LG4 and LG5 domains of the alpha chain?

The LN332 trimeric protein has a total molecular weight of approximately 628 kDa (as determined by non-reducing SDS-PAGE) and is represented by three individual bands (~367 kDa, 130 kDa, and 131 kDa) in reducing SDS-PAGE. While we can confirm that full-length DNA was transfected, since the protein is naturally expressed, we cannot definitively confirm whether the LG4 and LG5 domains are cleaved during protein maturation. However, our Biolaminin 332 product has been shown to support enhanced cell attachment and growth, demonstrating its functional effectiveness.