Why does the foam spontaneously detach the day after seeding or when I add the medium?

Foam detachment may occur due to one or more of the following reasons:

1. Non-hydrophobic surface: If the surface where the foam is placed is not hydrophobic, detachment can occur. Culture plates intended for suspension culture or non-tissue treated (non-TC) plates are typically hydrophobic. We recommend using plates from SARSTEDT, ref: 83.3922.500.
2. Small foam diameter: If the foam’s diameter is too small compared to its height, the contact area with the bottom may be insufficient. This can cause the foam to lift when medium is added. For a 20 µL foam, a diameter of 0.7-1 cm is optimal for good attachment.
3. Extended standing of Biosilk solution: If the Biosilk solution has been left at room temperature too long after thawing (more than 45-60 minutes), it may cause protein precipitation, resulting in a milky appearance. The solution should be used immediately after thawing.
4. Abrupt medium addition: Adding medium with excessive force can break or lift the foam. It is important to carefully first drop the medium around and then on top of the foam to prevent disruption.