Publications

Here is a selection of publications where different laminin isoforms are being used to create more authentic cell culture systems

  • Area of interest

  • Aligned Poly(ε-caprolactone) Nanofibers Guide the Orientation and Migration of Human Pluripotent Stem Cell-Derived Neurons, Astrocytes, and Oligodendrocyte Precursor Cells In Vitro

    Hyysalo A., Ristola M., Jpki T., Honkanen M., Vippola M., Narkilahti S.Macromolecular Bioscience, 2017

    Biomaterials can be used as supporting scaffolds, as they mimic the characteristics of the natural cellular environment. In this study, hPSC-derived neurons, astrocytes, and oligodendrocyte precursor cells (OPCs) are cultured on aligned poly(ε-caprolactone) nanofiber platforms, which guide cell orientation to resemble that of the spinal cord in vivo. Human neurons and astrocytes require extracellular matrix molecule coating for the nanofibers, but OPCs grow on nanofibers without additional treatment. Clinically relevant human recombinant laminin substrates (laminin-521 and laminin-511) are compatible with PCL nanofibers and can be used for efficient culturing of hPSC-derived neurons and astrocytes on PCL nanofibers. Furthermore, the nanofiber platform is combined with a 3D hydrogel scaffold with controlled thickness, and the nanofiber-mediated orientation of hPSC-derived neurons is also demonstrated in a 3D environment.

  • Laminin α5 substrates promote survival, network formation and functional development of human pluripotent stem cell-derived neurons in vitro

    Anu Hyysalo, Mervi Ristola, Meeri E.-L. Mäkinen, Sergei Häyrynen, Matti Nykter, Susanna Narkilahti. Stem Cell Research, 2017

    In this work, the authors compared different recombinant human laminin isoforms in the culture of hPSC derived neurons. They show that LN α5 chain is important for neuronal attachment, viability and network formation, and that neuronal functional development is enhanced by LN α5-substrates.

  • A defined xeno-free and feeder-free culture system for the derivation, expansion and direct differentiation of transgene-free patient-specific induced pluripotent stem cells

    Lu H.F., Chai C., Lim T.C., Leong M.F., Lim J.K., Gao S., Lim K.L., Wan A.C. Biomaterials 2014 

    Reprogramming of iPSCs on LN-521 and direct differentiation to dopaminergic cells on Laminin-521. This article demonstrates LN-521 as an optimal defined, xeno- and feeder-free matrix for the reprogramming of human iPS cells. Laminin-521 achieves high-efficiency reprogramming in different media, fast and easy expansion as well as direct differentiation to dopaminergic neurons on LN-521. The authors conclude that the efficient transgene-free hiPSC derivation and expansion on LN-521 enables clinical applications useful for human patient iPSCs and derivatives for cellular therapy.