Publications
Here is a selection of publications where different laminin isoforms were used to create more authentic cell culture systems.
Laminin-5 and type I collagen promote adhesion and osteogenic differentiation of animal serum-free expanded human mesenchymal stromal cells
Mittag F., Falkenberg E-M, Janczyk A., Götze M., Felka T., Aicher W.K. Kluba T.Orthopedic Reviews, 2012
In this article, the authors show that laminin 332 and type I collagen promote attachment and that laminin 332 promotes osteogenic differentiation of MSC. Expansion of MSC in animal serum-free, GMP-conforming media yielded vital cells meeting all minimal criteria for MSC. Attachment assay revealed a favorable binding of MSC to laminin 332 and type I collagen. Compared to plastic, osteogenic differentiation was significantly increased by laminin 332 after 28 days of culture, with no significant differences in gene expression patterns observed. Their data also confirm that laminin 332 serve better in bone repair, as this material promotes both firm attachment and osteogenic differentiation of MSC.
Osteoblast-derived Laminin-332 is a novel negative regulator of osteoclastogenesis in bone microenvironments
Uehara N., Kukita A., Kyumoto-Nakamura Y., Yamaza T., Yasuda H., Kukita T.Laboratory Investigation, 2017
In this article, the authors show that laminin 332 is expressed in primary osteoblasts, and is implicated in the regulation of osteoclast differentiation. Immunofluorescence analysis and RT-PCR analysis indicated specific expression of laminin 332 in osteoblast-like cells localized on the bone surface. Laminin 332 markedly inhibited osteoclastogenesis induced by receptor activator of nuclear factor kappa B (NF-κB) ligand (RANKL) when bone marrow-derived macrophages (BMMs) were cultured on laminin 332-coated plates. Laminin 332 also blocked RANKL-induced activation of mitogen-activated protein kinases (MAPKs) (ERK, JNK, and p38) and expression of NFATc1, c-Fos, and c-Jun. Laminin 332 suppressed osteoclast differentiation while retaining macrophage phenotypes, including nonspecific esterase activity and gene expression of lysozyme and EGF-like module-containing mucin-like hormone receptor-like 1 (Emr1). Furthermore, the treatment of primary osteoblasts with osteoclastogenic factors dramatically suppressed the expression of laminin 332. These findings suggest that laminin 332 produced by osteoblasts in bone tissues has a pivotal role in controlling normal bone remodeling through suppressing osteoclastogenesis.
The role of laminins in cartilaginous tissues: from development to regeneration
Sun Y., Wang TL., Toh WS, Pei M. Eur Cell Mater, 2017
In this review, the authors facilitate an understanding of the spatial and temporal interactions between cartilage-forming cells and laminin microenvironment to eventually advance cell-based cartilage engineering and regeneration. The expression of laminins in various developmental stages of cartilage and cartilage-like tissues, including developing, adult and pathological cartilage is being discussed. They delineated the expression of laminins in hyaline cartilage, fibrocartilage and cartilage-like tissue (nucleus pulposus) throughout several developmental stages. They also examined the effect of laminins on the biological activities of chondrocytes, including adhesion, migration, and survival. Furthermore, the potential influence of various laminin isoforms on cartilage-forming cells’ proliferation and chondrogenic differentiation was scrutinized.
Functionality of endothelial cells and pericytes from human pluripotent stem cells demonstrated in cultured vascular plexus and zebrafish xenografts
Valeria V Orlova, Yvette Drabsch, Christian Freund, Sandra Petrus-Reurer, Francijna E van den Hil, Suchitra Muenthaisong, Peter Ten Dijke, Christine L Mummery. Arterioscler Thromb Vasc Biol, 2014
This article describes simultaneous derivation of endothelial cells and pericytes from hiPSCs of different tissue origin.
Expression and function of laminins in the embryonic and mature vasculature
Rupert Hallmann, Nathalie Horn, Manuel Selg, Olaf Wendler, Friederike Pausch, Lydia M Sorokin. Physiol Rev, 2005
The endothelial cell basement membranes have been shown to be unique with respect to their laminin isoform composition. This review outlines the unique structural and functional features of vascular basement membranes, with focus on the endothelium and the laminin family of glycoproteins.
The extracellular matrix protein laminin α2 regulates the maturation and function of the blood-brain barrier
Michael J Menezes, Freyja K McClenahan, Cindy V Leiton, Azeez Aranmolate, Xiwei Shan, Holly Colognato. J Neurosc, 2014
In this article, Lama2(-/-) knock-out mice, lacking expression of the laminin α2 subunit of the laminin-211 heterotrimer expressed by astrocytes and pericytes, are shown to have defective blood-brain barrier (BBB ) in which systemically circulated tracer leaks into the brain parenchyma.
In vitro models of the blood-brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use
Hans C Helms, N Joan Abbott, Malgorzata Burek, Romeo Cecchelli, Pierre-Olivier Couraud, Maria A Deli, Carola Förster, Hans J Galla, Ignacio A Romero, Eric V Shusta, Matthew J Stebbins, Elodie Vandenhaute, Babette Weksler, Birger Brodin. J Cereb Blood Flow Metab, 2016
This review gives an overview of established in vitro blood-brain barrier models with a focus on their validation regarding a set of well-established blood-brain barrier characteristics. The authors also provide advantages and drawbacks of the different models described.
The adhesion GPCR GPR126 has distinct, domain-dependent functions in Schwann cell development mediated by interaction with Laminin-211
Petersen SC. et al.
In the peripheral nervous system, Schwann cell (SC) myelination requires the adhesion G protein-coupled receptor GPR126, which undergoes autoproteolytic cleavage into an N-terminal fragment (NTF) and a seven-transmembrane-containing C-terminal fragment (CTF). The authors noticed biphasic roles of GPR126 which were governed by interactions with Laminin-211 - a novel ligand for GPR126 that modulates receptor signaling via a tethered agonist. The work suggests a model in which Laminin-211 mediates GPR126-induced cAMP levels to control early and late stages of SC development.
α6β1 and α7β1 Integrins Are Required in Schwann Cells to Sort Axons
Marta Pellegatta, Adèle De Arcangelis, Alessandra D'Urso, Alessandro Nodari, Desirée Zambroni, Monica Ghidinelli, Vittoria Matafora, Courtney Williamson, Elisabeth Georges-Labouesse, Jordan Kreidberg, Ulrike Mayer, Karen K. McKee, Peter D. Yurchenco, Angelo Quattrini, Lawrence Wrabetz and Maria Laura Feltri. JNeurosci, 2013
In this article, the α subunits that form predominant laminin-binding β1 integrins were deleted in Schwann cells. The authors conclude that α6β1 and α7β1 are the laminin-binding integrins required for axonal sorting. The basal lamina of Schwann cells contains especially laminins 211, 411 and 511.
Laminin Alters Fyn Regulatory Mechanisms and Promotes Oligodendrocyte Development
Jenne Relucio, Iva D. Tzvetanova, Wei Ao, Sabine Lindquist and Holly Colognato. JNeurosci, 2009
The authors report that in laminin-deficient mice, oligodendrocyte progenitors accumulated inappropriately in adult brains. Laminin-211 was found to promote the transition of oligodendrocyte progenitors to newly formed oligodendrocytes. Laminin-enhanced differentiation was Src family kinase-dependent and resulted in the activation of the Src family kinase Fyn.