Publications

Here is a selection of publications where different laminin isoforms are being used to create more authentic cell culture systems

  • Area of interest

  • Dynamic blebbing: A bottleneck to human embryonic stem cell culture that can be overcome by Laminin-Integrin signaling

    Weng J-H., Cheung C., Talbot P.Stem Cell Research, 2018

    This study characterizes dynamic and apoptotic blebbing in human embryonic stem cells (hESC), identifies dynamic blebbing as a bottleneck to successful cell attachment during passaging, and demonstrates that dynamic blebbing can be rapidly stopped by plating cells on recombinant human laminin. In freshly plated hESC, dynamic and apoptotic blebbing differed in time of occurrence, bleb retraction rate, mitochondrial membrane potential, and caspase 3&7 activation. While dynamic blebbing can be controlled with drugs that inhibit myosin II, these methods have off-target effects and are not suitable for clinical applications. Recombinant human laminin-521 or addition of laminin-111 to Matrigel provided a safe method to drastically decrease dynamic blebbing and improve cell attachment with proteins normally found in the inner cell mass. Inhibition of focal adhesion kinase, which is activated by binding of integrins to laminin, prolonged dynamic blebbing and inhibited attachment. These data show that hESC binds rapidly to laminins through an integrin, which activates focal adhesion kinase that in turn downregulates dynamic blebbing. Laminins enabled hESC to rapidly attach during passaging, improved plating efficiency, enabled passaging of single pluripotent stem cells, and avoided the use of inhibitors that have non-specific off-target effects. These data provide a strategy for improving hESC culture using biologically safe recombinant human proteins.

  • Laminin-511 is an epithelial message promoting dermal papilla development and function during early hair morphogenesis

    Jing Gao, Mindy C. DeRouen, Chih-Hsin Chen, Michael Nguyen, Ngon T. Nguyen, Hiroyuki Ido, Kenji Harada, Kiyotoshi Sekiguchi, Bruce A. Morgan, Jeffery H. Miner, Anthony E. Oro, and M. Peter Marinkovich. Genes Dev, 2008

    Abnormal adhesion of grafted corneal endothelial cells (CECs) affects the application of intracameral injection for corneal endothelial dysfunction therapy. The authors explored whether laminin 511 (LN511) improves the therapeutic function of the intracameral CEC injection. Injected LN511 was found to be able to settle and form a coating on the posterior surface of Descemet's membrane (DM). The data suggests that the strategy of LN511 precoating and CECs' intracameral injection could be a potential method for the therapy of corneal endothelial dysfunction.

  • Laminin-10 is crucial for hair morphogenesis

    Jie Li, Julia Tzu, Yi Chen, Yan-Ping Zhang, Ngon T Nguyen, Jing Gao, Maria Bradley, Douglas R Keene, Anthony E Oro, Jeffrey H Miner, M Peter Marinkovich. EMBO J, 2003

    This article describes the essential role of laminin-10 (laminin-511) in hair follicle development.