Publications
Here is a selection of publications where different laminin isoforms were used to create more authentic cell culture systems.
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Structural biology of laminins
Hohenester E. Essays in Biochemistry, 2019
This article is a historical review on the structural biology of laminin proteins.
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Regulation of the Immune System by Laminins
Simon T., Bromberg J. Trends Immunol, 2017
This review summarizes the structure of laminins, the modulation of their expression, and their interactions with the immune system. In addition, the role of laminins in autoimmune diseases and transplantation are discussed.
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A Chemically Defined Hydrogel for Human Liver Organoid Culture
Ye S., Boeter J.W.B., Mihajlovic M., van Steenbeek F.G, van Wolferen M.E., Oosterhoff L.A., Marsee A., Caiazzo M., van der Laan L.J.W., Penning L.C., Vermonden T., Spee B., and Schneeberger K. Adv. Funct. Mater. 2020
Here, a novel hydrogel-based on polyisocyanopeptides (PIC) and Biolaminin-111 is described for human liver organoid cultures. PIC is a synthetic polymer that can form a hydrogel with thermosensitive properties, making it easy to handle and very attractive for clinical applications. PIC hydrogel alone was not sufficient to support organoid growth. The addition of a laminin-entactin complex (LEC) to the plain PIC gel, resulted in efficient organoid formation and proliferation that seemed comparable to the Matrigel controls, with lower stiffnesses most favorable for organoid proliferation. The stem cell phenotype and proliferation and differentiation capacity of the organoids could be maintained in PIC-LEC over several passages, enabling their seemingly unlimited expansion and subsequent maturation. Moreover, organoids can be efficiently differentiated toward a hepatocyte-like phenotype with key liver functions. Importantly, they also show that the LEC in the PIC-LEC gels could be replaced by Biolaminin-111, resulting in a completely synthetic hydrogel for the expansion of human liver organoids.
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The ability of inner-cell-mass cells to self-renew as embryonic stem cells is acquired following epiblast specification
Boroviak T., Loos R., Bertone P., Smith A. and Nichols J.Nature Cell Biology, 2014
The authors show that mouse ICM cells from early blastocysts can progress to ERK independence if provided with a specific laminin substrate. We show by RNA sequencing that Laminin-511 is expressed in the early ICM, as is B1-integrin, which mediates laminin binding in ESCs. No expression of Laminin-521 in mouse ICM. Single ESC derivation in 2i-LIF on a fibronectin-LN511 mix and clonal ESC lines expansion on LN511.
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A Balance between Secreted Inhibitors and Edge Sensing Controls Gastruloid Self-Organization
Etoc F., Metzger J., Ruzo A., Kirst C., Yoney A., Ozair Z.M, Brivanlou A.H., Siggia E.D. Developmental cell, 2016
Used colonies of hESC grown on the micropatterned substrate and differentiated with BMP4 to model patterning events in the human embryo. Coated Costar Transwells with LN521.
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CCR2+CCR5+ T Cells Producing Matrix Metalloproteinase-9 and Osteopontin in the Pathogenesis of Multiple Sclerosis
Sato W., Tomita A., Ichikawa D., Lin Y., Kishida H., Miyake S., Ogawa M., Okamoto T., Murata M., Kuroiwa Y., Aranami T., Yamamura T. Journal of Immunology, 2012
The authors coated the upper sides of Transwell membrane inserts with Biolaminin 211. The aim was to recapitulate the glia limitans layered with parenchymal basal lamina experimentally, to study how effectively T-cells invade accross the physiological barrier separating CSF from the CNS parenchyma. Normal human astrocytes were seeded on the lower sides of the membrane inserts. T-cell migration across the astrocyte layered with laminin was less efficient and thus, this model would exhibit barrier functions against the penetration of activated T cells.
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Early embryonic-like cells are induced by downregulating replication-dependent chromatin assembly
Ishiuchi T., Enriquez-Gasca R., Mizutani E., Bošković A., Ziegler-Birling C., Rodriguez-Terrones D., Wakayama T., Vaquerizas J.M., Torres-Padilla M-E.Nature structural & molecular biology, 2015
mES cells cultured on coverslips coated with laminin-511for an RNA-FISH assay.
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Dynamic Heterogeneity and DNA Methylation in Embryonic Stem Cells
Singer Z.S., Yong J., Tischler J., Hackett J.A., Altinok A., Surani M.A., Cai L., and Elowitz M.B.Cell Press, 2014
Analyzis of gene expression in individual ESCs using single-molecule RNA-FISH and quantitative time-lapse movies. Fluorescence Time-Lapse Microscopy and Data analysis on reporter cells mixed with unlabeled parental cells at 1:9 ratio and plated at a total density of 20,000 cells/cm2 on glass-bottom plates (MatTek) coated with human laminin-511 >12 hr before imaging.
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Control of ground-state pluripotency by allelic regulation of Nanog
Miyanari Y., Torres-Padilla M.E.Nature Letter, 2012
Laminin-511 used to coat glass-bottomed dishes.
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Live visualization of chromatin dynamics with fluorescent TALEs
Miyanari Y., Ziegler-Birling C., Torres-Padilla M.E.Nature structural & molecular biology, 2013
The authors of these two Nature publications show that laminin can be coated directly on glass, which many other substrates and proteins can not. This enables the growth of pluripotent stem cells as monolayers even on glass, which is especially suitable for live imaging.