Human recombinant laminins

Not sure which laminin isoform works best for your cells?

Choose four different Biolaminin LN products (100 μg) and pay only for three. This allows you to test which Biolaminin substrate is optimal for your cell culture.

Recreating the natural cell niche is key to successful cultivation

Robust cell culture is achieved by culturing cells on their natural cell adhesion molecule, laminin. All cells in the body make close contact with the extracellular matrix and one component, the basement membrane, surrounds all organs and functional units in vivo. The basement membrane is important for cell anchorage, survival, migration, differentiation, and function.

There are many proteins in the basement membrane, but laminins are tissue-specific and play major roles in creating and sustaining cellular niches.

To date, more than 16 different laminin isoforms have been described. Through studies of human diseases and gene deletion experiments, the functions of a few isoforms have been elucidated. However, the exact role of most isoforms, as well as the composition of laminins in different cell niches, is still unknown.

In order to maintain the proper function of cells and to differentiate them towards desired cell phenotypes, correct replication of the laminin foundation in the in vivo cell niche is essential.

LAMscreen makes it easy for you to test which Biolaminin(s) provides the best support for your cells in vitro

At least the following laminin isoforms have been demonstrated to serve critical roles for the listed cell types:

Biolaminin 521 LN

Single-cell human ES/iPS cell culture (Rodin, 2014)
Clonal pluripotent stem cell culture (Rodin, 2014)
Podocytes (Miner, 2011)

Biolaminin 511 LN

Culture of human ES/iPS cells (Rodin, 2010)
RPE-cells culture (Aisenbrey, 2006)
Hair follicles (Li, 2003)
Certain neuronal cells (Fusaoka-Nishioka, 2011)

Biolaminin 421 LN

Mesangial cell culture (Abrass, 2010)
Kidney microvasculature (Thyboll, 2002)
Motorneuron synaptic transmission (Patton, 2001)

Biolaminin 411 LN

Endothelial cells (Hallmann, 2005)
Insulin-producing ß-cells (Nikolova, 2006)

Biolaminin 332 LN

Keratinocytes (Ghohestani, 2001)

Biolaminin 221 LN

Skeletal muscle (Patton, 1999)
Heart muscle tissue (Ja, 2016)

Biolaminin 211 LN

Skeletal muscle (Patton, 1999)
Smooth muscle (Relan, 1999)
Certain neurons (Lathia, 2007)
Heart muscle tissue (Ja, 2016)

Biolaminin 121 LN

ES/iPS differentiation (Miner, 2004)
Neurite outgrowth (Sasaki, 2010)

Biolaminin 111 LN

ES/iPS differentiation (Miner, 2004)

Read more on the cell type specific applications to help you in choosing the right laminins for your culture system.

  • 3D culture

    Biosilk and Biolaminin key advantages 3D cell culture and organoid formation 3D culture systems for disease modeling, drug screening, and […]
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  • Cancer cells

    Biorelevant culture of cancer stem cells on Biolaminin substrates Cancer cells use laminin/integrin signaling pathways that promote tumor cell growth, invasion, and […]
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  • Pancreatic cells

    Biorelevant culture of pancreatic cells on Biolaminin substrates The basement membrane formed next to the beta-cells contains alpha-4 and alpha-5 laminin […]
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  • Lung cells

    Biorelevant culture of lung cells on Biolaminin substrates Laminin expression in lung Laminin is key proteins in the basement membranes of the airway […]
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  • Mammary gland cells

    Biorelevant culture of human mammary cells on Biolaminin substrates Laminins have both overlapping and unique functions in the mammary gland tissue Mammary […]
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  • Skin cells

    Biorelevant culture of keratinocytes on Biolaminin substrates The basement membrane composition in the epidermis Human epidermal keratinocyte (HEK) cells are […]
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  • Eye cells

    Biorelevant culture of eye cells Laminin proteins are important components in the eye microenvironment Biolaminin products have been succesfully used […]
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Size guide

Not sure how much laminin you need? To make it easy, we have created a tool where you can calculate the amount needed for your experiments. Just choose the culture well format and fill in the desired coating concentration to see the amount required.