Publications
Here is a selection of publications where different laminin isoforms were used to create more authentic cell culture systems.
Osteoblast-derived Laminin-332 is a novel negative regulator of osteoclastogenesis in bone microenvironments
Uehara N., Kukita A., Kyumoto-Nakamura Y., Yamaza T., Yasuda H., Kukita T.Laboratory Investigation, 2017
In this article, the authors show that laminin 332 is expressed in primary osteoblasts, and is implicated in the regulation of osteoclast differentiation. Immunofluorescence analysis and RT-PCR analysis indicated specific expression of laminin 332 in osteoblast-like cells localized on the bone surface. Laminin 332 markedly inhibited osteoclastogenesis induced by receptor activator of nuclear factor kappa B (NF-κB) ligand (RANKL) when bone marrow-derived macrophages (BMMs) were cultured on laminin 332-coated plates. Laminin 332 also blocked RANKL-induced activation of mitogen-activated protein kinases (MAPKs) (ERK, JNK, and p38) and expression of NFATc1, c-Fos, and c-Jun. Laminin 332 suppressed osteoclast differentiation while retaining macrophage phenotypes, including nonspecific esterase activity and gene expression of lysozyme and EGF-like module-containing mucin-like hormone receptor-like 1 (Emr1). Furthermore, the treatment of primary osteoblasts with osteoclastogenic factors dramatically suppressed the expression of laminin 332. These findings suggest that laminin 332 produced by osteoblasts in bone tissues has a pivotal role in controlling normal bone remodeling through suppressing osteoclastogenesis.
The role of laminins in cartilaginous tissues: from development to regeneration
Sun Y., Wang TL., Toh WS, Pei M. Eur Cell Mater, 2017
In this review, the authors facilitate an understanding of the spatial and temporal interactions between cartilage-forming cells and laminin microenvironment to eventually advance cell-based cartilage engineering and regeneration. The expression of laminins in various developmental stages of cartilage and cartilage-like tissues, including developing, adult and pathological cartilage is being discussed. They delineated the expression of laminins in hyaline cartilage, fibrocartilage and cartilage-like tissue (nucleus pulposus) throughout several developmental stages. They also examined the effect of laminins on the biological activities of chondrocytes, including adhesion, migration, and survival. Furthermore, the potential influence of various laminin isoforms on cartilage-forming cells’ proliferation and chondrogenic differentiation was scrutinized.