Publications
Here is a selection of publications where different laminin isoforms were used to create more authentic cell culture systems.
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Recent studies show why stem cells need full-length laminin substrate
Recent articles highlighting human embryogenesis and intact laminin functionality show why stem cells prefer full-length laminin-521 substrate.
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Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs. npj Regenerative Medicine, 2023
Lynn Yap, Li Yen Chong, Clarissa Tan, Swarnaseetha Adusumalli, Millie Seow, Jing Guo, Zuhua Cai, Sze Jie Loo, Eric Lim, Ru San Tan, Elina Grishina, Poh Loong Soong, Narayan Lath, Lei Ye, Enrico Petretto & Karl Tryggvason.
Dr. Lynn Yap and colleagues present pre-clinical evidence of their promising myocardial infarction treatment using stem cell-derived committed cardi […]
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Observing Dynamic Conformational Changes within the Coiled-Coil Domain of Different Laminin Isoforms Using High-Speed Atomic Force Microscopy
Akter L, Flechsig H, Marchesi A, and Franz CM. Int. J. Mol. Sci. 2024
This study by Akter and colleagues provides a visual insight into the significance of using whole, full-length laminin to mimic biological condition […]
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Integration of xeno-free single-cell cloning in CRISPR-mediated DNA editing of human iPSCs improves homogeneity and methodological efficiency of cellular disease modeling
Atefeh Namipashaki, Kealan Pugsley, Xiaodong Liu, Kirra Abrehart, Sue Mei Lim, Guizhi Sun, Marco J. Herold, Jose M. Polo, Mark A. Bellgrove, and Ziarih Hawi. Stem Cell Reports, 2023
This article presents an improved method for generating genetically homogeneous induced pluripotent stem cell (iPSC) clones following CRISPR-Cas9 ge […]
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Leveraging interacting signaling pathways to robustly improve the quality and yield of human pluripotent stem cell-derived hepatoblasts and hepatocytes
Claudia Raggi, Marie-Agnès M’Callum, Quang Toan Pham, Perrine Gaub, Silvia Selleri, Nissan Vida Baratang, Chenicka Lyn Mangahas, Gaël Cagnone, Bruno Reversade, Jean-Sébastien Joyal, Massimiliano Paganelli. Stem Cell Reports, 2022
This study describes a robust and scalable protocol for pluripotent stem cell (PSC) differentiation into high-quality bipotent hepatoblasts and hepa […]
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Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro
Javier Calvo-Garrido, Dania Winn, Camilla Maffezzini, Anna Wedell, Christoph Freyer, Anna Falk, Anna Wredenberg. STAR Protocols, 2021
This protocol describes the derivation of neuroepithelial stem (NES) cells from human induced pluripotent stem cells. NES cells can be further diffe […]
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CRISPRi-mediated transcriptional silencing in iPSCs for the study of human brain development
Pia Annette Johansson, Anita Adami, Johan Jakobsson. STAR Protocols, 2022
This protocol describes using CRISPRi-mediated transcriptional silencing in human induced pluripotent stem cells. In addition, it contains an effici […]
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Generation of a CHIP isogenic human iPSC-derived cortical neuron model for functional proteomics
Catarina Dias, Erisa Nita. Jakub Faktor, Lenka Hernychova, Tilo Kunath, Kathryn L. Ball. STAR Protocols, 2022
This protocol describes the production of gene-edited cells using CRISPR-Cas9 and a patient-derived induced pluripotent stem cell (iPSC) line. Biola […]
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Methods for Automated Single Cell Isolation and Sub-Cloning of Human Pluripotent Stem Cells
Valeria Fernandez Vallone, Narasimha Swamy Telugu, Iris Fischer,Duncan Miller, Sandra Schommer, Sebastian Diecke, Harald Stachelscheid. Current Protocols in Stem Cell Biology, 2020
This publication describes automated workflows to facilitate high-throughput hPSC clonal selection and expansion, which is required for example afte […]
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Astrocytic laminin-211 drives disseminated breast tumor cell dormancy in brain
Jinxiang Dai, Patrick J. Cimino, Kenneth H. Gouin III, Candice A. Grzelak, Alexander Barrett, Andrea R. Lim, Annalyssa Long, Stephanie Weaver, Lindsey T. Saldin, Aiyedun Uzamere, Vera Schulte, Nigel Clegg, Laura Pisarsky, David Lyden, Mina J. Bissell, Simon Knott, Alana L. Welm, Jason H. Bielas, Kirk C. Hansen, Frank Winkler, Eric C. Holland and Cyrus M. Ghajar. Nature Cancer, 2022
This study shows that astrocyte-deposited laminin-211 drives disseminated tumor cell (DTC) quiescence in brain metastases by inducing the dystroglyc […]
