Publications

Here is a selection of publications where different laminin isoforms are being used to create more authentic cell culture systems

  • Area of interest

  • Laminin-511 is an epithelial message promoting dermal papilla development and function during early hair morphogenesis

    Jing Gao, Mindy C. DeRouen, Chih-Hsin Chen, Michael Nguyen, Ngon T. Nguyen, Hiroyuki Ido, Kenji Harada, Kiyotoshi Sekiguchi, Bruce A. Morgan, Jeffery H. Miner, Anthony E. Oro, and M. Peter Marinkovich. Genes Dev, 2008

    Abnormal adhesion of grafted corneal endothelial cells (CECs) affects the application of intracameral injection for corneal endothelial dysfunction therapy. The authors explored whether laminin 511 (LN511) improves the therapeutic function of the intracameral CEC injection. Injected LN511 was found to be able to settle and form a coating on the posterior surface of Descemet's membrane (DM). The data suggests that the strategy of LN511 precoating and CECs' intracameral injection could be a potential method for the therapy of corneal endothelial dysfunction.

  • Laminin 511 Precoating Promotes the Functional Recovery of Transplanted Corneal Endothelial Cells

    Can Zhao, Qingjun Zhou, Haoyun Duan, Xin Wang, Yanni Jia, Yajie Gong, Wenjing Li, Chunxiao Dong, Zongyi Li, and Weiyun Shi. Tissue Eng Part A, 2020

    Abnormal adhesion of grafted corneal endothelial cells (CECs) affects the application of intracameral injection for corneal endothelial dysfunction therapy. The authors explored whether laminin 511 (LN511) improves the therapeutic function of the intracameral CEC injection. Injected LN511 was found to be able to settle and form a coating on the posterior surface of Descemet's membrane (DM). The data suggests that the strategy of LN511 precoating and CECs' intracameral injection could be a potential method for the therapy of corneal endothelial dysfunction.

  • Laminin-332 coordinates mechanotransduction and growth cone bifurcation in sensory neurons

    Li-Yang Chiang, Kate Poole, Beatriz E Oliveira, Neuza Duarte, Yinth Andrea Bernal Sierra, Leena Bruckner-Tuderman, Manuel Koch, Jing Hu, Gary R Lewin. Nat Neurosci, 2011

    The authors found that mouse epidermal keratinocytes produce a matrix that is inhibitory for sensory mechanotransduction and that the active molecular component is laminin-332. Laminin-332 could locally control sensory axon branching behavior, and the loss of laminin-332 in humans led to increased sensory terminal branching and may lead to a de-repression of mechanosensitive currents.

  • Laminin-10 is crucial for hair morphogenesis

    Jie Li, Julia Tzu, Yi Chen, Yan-Ping Zhang, Ngon T Nguyen, Jing Gao, Maria Bradley, Douglas R Keene, Anthony E Oro, Jeffrey H Miner, M Peter Marinkovich. EMBO J, 2003

    This article describes the essential role of laminin-10 (laminin-511) in hair follicle development.

  • Human amnion contains a novel laminin variant, laminin 7, which like laminin 6, covalently associates with laminin 5 to promote stable epithelial-stromal attachment

    M F Champliaud, G P Lunstrum, P Rousselle, T Nishiyama, D R Keene, R E Burgeson. J Cell Biol, 1996

    This article describes examination of laminin 5 (laminin-332) in amnion tissue, and show potential interaction with laminin-6 (311) and a new variant laminin-7 (321). Amniotic epithelial basement membrane is suggested to be similar with that of skin.

  • Agitation conditions for the culture and detachment of hMSCs from microcarriers in multiple bioreactor platforms

    Alvin W. Nienow, Christopher J. Hewitt, Thomas R.J. Heathman, Veronica A.M. Glyn, Gonҫalo N. Fonte, Mariana P. Hanga, Karen Coopman, Qasim A. Rafiq. Biochemical Engineering Journal, 2016

    In this article, 22 combinations of conditions in which hMSCs have been cultivated on microcarriers are reported - four different types and sizes of bioreactor. Biolaminin-521 surface coating was applied in two of them. The agitation strategy described is suggested to offer a basis for scale-up.

  • The role of adipose protein derived hydrogels in adipogenesis

    Shiri Uriel, Jung-Ju Huang, Monica L Moya, Megan E Francis, Rui Wang, Shu-Ying Chang, Ming-Huei Cheng, Eric M Brey. Biomaterials, 2008

    This study investigated the potential of adipose-derived matrices to induce adipogenesis in vitro and in vivo. Solutions containing basement membrane proteins and growth factors were extracted from subcutaneous adipose tissue and were induced to form gels. The authors suggested potential use for adipose tissue engineering.

  • Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures

    Tanja Hyvärinen, Anu Hyysalo, Fikret Emre Kapucu, Laura Aarnos, Andrey Vinogradov, Stephen J Eglen, Laura Ylä-Outinen, Susanna Narkilahti. Sci Rep, 2019

    In this article, differentiation of functionally active hPSC-derived cortical networks on defined laminin-521 substrate is reported. They assessed compared the activity development of hPSC-derived networks to that of widely used rat embryonic cortical cultures using microelectrode array (MEA) measurements. The authors conclude that hPSC-derived neural cultures produced with a defined protocol generate cortical type network activity, and they could be applied as a human-specific model for pharmacological studies and modeling network dysfunctions.

  • Assembly of functionalized silk together with cells to obtain proliferative 3D cultures integrated in a network of ECM-like microfibers

    Ulrika Johansson, Mona Widhe, Nancy Dekki Shalaly, Irene Linares Arregui, Linnea Nilebäck, Christos Panagiotis Tasiopoulos, Carolina Åstrand, Per-Olof Berggren, Christian Gasser, My Hedhammar. Sci Rep, 2019

    This article describes the use of fibrous spider silk 3D network (Biosilk) for the culture of mammalian cells. The cells get uniformly integrated between the formed microfibers and the cells are highly proliferative, spreading out more efficiently than when encapsulated in a hydrogel. The authors conclude that the silk-assembly in presence of cells constitutes a viable option for 3D culture of cells integrated in an ECM-like network.

  • Efficiently Specified Ventral Midbrain Dopamine Neurons from Human Pluripotent Stem Cells Under Xeno-Free Conditions Restore Motor Deficits in Parkinsonian Rodents

    Jonathan C Niclis, Carlos W Gantner, Walaa F Alsanie, Stuart J McDougall, Chris R Bye, Andrew G Elefanty, Edouard G Stanley, John M Haynes, Colin W Pouton, Lachlan H Thompson, Clare L Parish. Stem Cells Transl Med, 2017

    The authors describe the first fully defined feeder- and xenogeneic-free protocol for the generation of vmDA neurons from hPSCs for use in animal models of Parkinson's disease. This protocol, utilizing Biolaminin 521 as a component, consistently increases both the yield and proportion of vmDA neural progenitors and neurons that display classical vmDA metabolic and electrophysiological properties. These findings may help in translation of hPSC-derived neurons into the clinic.