Publications
Here is a selection of publications where different laminin isoforms are being used to create more authentic cell culture systems
Protocol for automated production of human stem cell derived liver spheres
Jose Meseguer-Ripolles, Alvile Kasarinaite, Baltasar Lucendo-Villarin, David C Hay. STAR Protoc, 2021
In this article, the authors describe how they produce human liver spheres from pluripotent stem cell-derived hepatic progenitors, endothelial cells, and hepatic stellate cells, using LN521 in the differentiation protocol. Their process is automated using liquid handling and pipetting systems, permitting cost-effective scale-up and reducing sphere variability.
Assembly of FN-silk with laminin-521 to integrate hPSCs into a three-dimensional culture for neural differentiation
Carolina Åstrand, Veronique Chotteau, Anna Falk, My Hedhammar Biomater Sci, 2020
This article describes the use of a recombinant spider silk protein functionalized with a cell binding motif from fibronectin in combination with a human recombinant laminin 521 (LN-521) to create a fully defined stem cell niche in 3D. The results show that hPSCs integrated into the foam develop into neural progenitors and that they stay viable during long-term differentiations. The culture system also supports morphogenesis mimicking the human brain development and can serve as base for engineering of hPSC-derived neural tissue. The article describes the 3D culture matrix sold under the name Biosilk®.
Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial Progenitor Cells into Midbrain Dopaminergic Neurons
Nolbrant S., Giacomoni J., Hoban D.B, Bruzelius A., Birtele M., Chandler-Militello D., Pereira M., Rylander Ottosson D., Goldman S.A., Parmar M. Stem Cell Reports, 2020
Human glial progenitor cells (hGPCs) are promising cellular substrates to explore for the in situ production of new neurons for brain repair. Proof of concept for direct neuronal reprogramming of glial progenitors using human cells has been difficult to perform since hGPCs are born late during human fetal development, with limited accessibility for in vitro culture. In this study, the authors provide evidence that hGPCs isolated from both the human fetal brain and differentiated from hESCs can be successfully reprogrammed into functional iNs, including induced DA neurons (iDANs). They also establish a renewable and reproducible stem cell-based hGPC system for direct neural conversion in vitro. Using this system, they have identified optimal combinations of fate determinants for the efficient dopaminergic (DA) conversion of hGPCs, thereby yielding a therapeutically relevant cell type that selectively degenerates in Parkinson’s disease.
Laminin 511 Precoating Promotes the Functional Recovery of Transplanted Corneal Endothelial Cells
Can Zhao, Qingjun Zhou, Haoyun Duan, Xin Wang, Yanni Jia, Yajie Gong, Wenjing Li, Chunxiao Dong, Zongyi Li, and Weiyun Shi. Tissue Eng Part A, 2020
Abnormal adhesion of grafted corneal endothelial cells (CECs) affects the application of intracameral injection for corneal endothelial dysfunction therapy. The authors explored whether laminin 511 (LN511) improves the therapeutic function of the intracameral CEC injection. Injected LN511 was found to be able to settle and form a coating on the posterior surface of Descemet's membrane (DM). The data suggests that the strategy of LN511 precoating and CECs' intracameral injection could be a potential method for the therapy of corneal endothelial dysfunction.
Laminin-332 coordinates mechanotransduction and growth cone bifurcation in sensory neurons
Li-Yang Chiang, Kate Poole, Beatriz E Oliveira, Neuza Duarte, Yinth Andrea Bernal Sierra, Leena Bruckner-Tuderman, Manuel Koch, Jing Hu, Gary R Lewin. Nat Neurosci, 2011
The authors found that mouse epidermal keratinocytes produce a matrix that is inhibitory for sensory mechanotransduction and that the active molecular component is laminin-332. Laminin-332 could locally control sensory axon branching behavior, and the loss of laminin-332 in humans led to increased sensory terminal branching and may lead to a de-repression of mechanosensitive currents.
Human amnion contains a novel laminin variant, laminin 7, which like laminin 6, covalently associates with laminin 5 to promote stable epithelial-stromal attachment
M F Champliaud, G P Lunstrum, P Rousselle, T Nishiyama, D R Keene, R E Burgeson. J Cell Biol, 1996
This article describes examination of laminin 5 (laminin-332) in amnion tissue, and show potential interaction with laminin-6 (311) and a new variant laminin-7 (321). Amniotic epithelial basement membrane is suggested to be similar with that of skin.
Agitation conditions for the culture and detachment of hMSCs from microcarriers in multiple bioreactor platforms
Alvin W. Nienow, Christopher J. Hewitt, Thomas R.J. Heathman, Veronica A.M. Glyn, Gonҫalo N. Fonte, Mariana P. Hanga, Karen Coopman, Qasim A. Rafiq. Biochemical Engineering Journal, 2016
In this article, 22 combinations of conditions in which hMSCs have been cultivated on microcarriers are reported - four different types and sizes of bioreactor. Biolaminin-521 surface coating was applied in two of them. The agitation strategy described is suggested to offer a basis for scale-up.
The role of adipose protein derived hydrogels in adipogenesis
Shiri Uriel, Jung-Ju Huang, Monica L Moya, Megan E Francis, Rui Wang, Shu-Ying Chang, Ming-Huei Cheng, Eric M Brey. Biomaterials, 2008
This study investigated the potential of adipose-derived matrices to induce adipogenesis in vitro and in vivo. Solutions containing basement membrane proteins and growth factors were extracted from subcutaneous adipose tissue and were induced to form gels. The authors suggested potential use for adipose tissue engineering.
Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures
Tanja Hyvärinen, Anu Hyysalo, Fikret Emre Kapucu, Laura Aarnos, Andrey Vinogradov, Stephen J Eglen, Laura Ylä-Outinen, Susanna Narkilahti. Sci Rep, 2019
In this article, differentiation of functionally active hPSC-derived cortical networks on defined laminin-521 substrate is reported. They assessed compared the activity development of hPSC-derived networks to that of widely used rat embryonic cortical cultures using microelectrode array (MEA) measurements. The authors conclude that hPSC-derived neural cultures produced with a defined protocol generate cortical type network activity, and they could be applied as a human-specific model for pharmacological studies and modeling network dysfunctions.
Assembly of functionalized silk together with cells to obtain proliferative 3D cultures integrated in a network of ECM-like microfibers
Ulrika Johansson, Mona Widhe, Nancy Dekki Shalaly, Irene Linares Arregui, Linnea Nilebäck, Christos Panagiotis Tasiopoulos, Carolina Åstrand, Per-Olof Berggren, Christian Gasser, My Hedhammar. Sci Rep, 2019
This article describes the use of fibrous spider silk 3D network (Biosilk) for the culture of mammalian cells. The cells get uniformly integrated between the formed microfibers and the cells are highly proliferative, spreading out more efficiently than when encapsulated in a hydrogel. The authors conclude that the silk-assembly in presence of cells constitutes a viable option for 3D culture of cells integrated in an ECM-like network.
