Biorelevant culture of endothelial cells on Biolaminin substrates
Laminin-521 can be used as an optimal matrix for primary vascular endothelial cells
Endothelial cells making up the vascular network in all organs of the body express and secrete laminin-411 and laminin-511 (Hallman et al., 2005). During angiogenesis, laminins are produced and deposited from the stalk cells to the tip cell filopodia (Jakobsson, 2008) and laminin-521 was recently shown to be crucial for retinal angiogenesis (Gnanaguru et al., 2013).
Mature endothelial cells are often grown on fibronectin and this results in a de-differentiation of cells within hours to days that can be seen by complete loss of specific endothelial markers such as the von Willenbrandt factor (vWF). However, if instead of growing primary endothelial cells, such as human umbilical cord endothelial cells (HUVECs), on laminin-521 or laminin-511 or laminin-411 we have successfully been able to culture HUVECs for as long as 20 passages with continuously strong expression of vWF (unpublished data).
Laminins are efficient coating substances that improve the yield of in vitro corneal endothelial cell cultures
Corneal endothelial cells (CECs) are highly polarized flat cells that form a uniform monolayer of the innermost layer of the cornea, separating the cornea from the aqueous humor that fills the anterior chamber. CECs plays several essential roles in corneal homeostasis and are specialized in regulating corneal hydration and transparency.
The basal surface of the CSCs rest on an amorphous collagenous membrane, Descemet’s membrane, composed of type IV collagen, type VIII collagen, perlecan, nidogens, laminin-332, laminin-411, and laminin-511 (Kabosova et al., 2007). Integrin α3β1 is the only protein found exclusively at the basal surface of the CSCs, forming an almost homogenous layer (He et al., 2016) and ligands of integrin α3β1, such as laminin-332, laminin-511, and laminin-521 are efficient coating substances that improve the yield of in vitro CEC cultures.
Laminin-521 and -511 for the isolation and expansion of corneal endothelial progenitors
Corneal endothelial progenitors are efficiently isolated and expanded on laminin-521 and laminin-511 (Hara et al., 2014). Hara and colleagues demonstrate that the proliferative capacity of these endothelial progenitors is very high on laminin-511 compared to conventional methods. Laminin-511 can thus be used to rapidly isolate and expand a homogenous population of endothelial progenitors that can be differentiated to endothelial cells in a serum-free environment.
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Differentiation of Human Embryonic Stem Cells to Endothelial Progenitor Cells on Laminins in Defined and Xeno-free Systems
Nguyen M.T.X., Okina E., Chai X., Tan K.H., Hovatta O., Ghosh S., Tryggvason K. Stem Cell Reports, 2016
Instructions: Coating with Biolaminin substrates
Protocol and concentration calculations for coating cultureware with Biolaminin
Biolaminin Key Advantages
Biolaminin 521 improves endothelial progenitor proliferation. Biolaminin 521 also helps to maintain cell identity in primary endothelial cell culture.
Specific laminin isoforms are present in different tissue microenvironments and they are essential for cell survival, proliferation and differentiation. Biolaminin products allow you to imitate the natural cell-matrix interactions in vitro.
Our products have consistent composition and quality. This enables minimized variability between experiments and uniform pluripotency gene expression profiles between different cell lines.
All our matrices are chemically defined and animal origin-free, which makes them ideal substrates for each level of the scientific process – from basic research to clinical applications.
Numerous scientists have found our products and finally succeeded in their specific stem cell application. The power of full-length laminins incorporated into various cell systems is well documented in scientific articles and clinical trials.
Biolaminin 521 LN (LN521)
Human recombinant laminin 521Biolaminin 521 LN is the natural laminin for pluripotent stem cells and therefore reliably facilitates self-renewal of human ES and iPS cells in a chemically defined, feeder-free and animal origin-free stem cell culture system. LN521 is animal origin-free to the primary level.VIEW product
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