After seeding cells on Biolaminin, I experience an uneven spread. What could be the reason for that?

An uneven cell spread is often due to insufficient coating and may result from the following:

1. Insufficient coating concentration

• A concentration that is too low may fail to support even cell growth.
• Solution: Increase the coating concentration until it provides adequate support for uniform cell growth..

2. Poor coating coverage or plate drying

• Ensure the entire surface is evenly covered with the laminin coating solution when preparing fresh plates.
• Avoid letting the plate dry out, as this will inactivate the laminin coating.
• Long incubation times or prolonged storage without proper sealing can lead to evaporation, causing parts of the plate (often the center) to dry out.

By addressing these factors, you can achieve consistent cell spreading and optimal experimental outcomes.