Can Biolaminin 521 support clump passaging of stem cells?

Yes, Biolaminin 521 supports both single-cell and clump passaging of stem cells. However, we recommend single-cell passaging or passaging small clumps, as it is a more reliable method that allows for standardization. This approach ensures that each cell has equal contact with the coating and medium, promoting a homogeneous environment. Biolaminin 521 serves as the natural niche protein for the cells and facilitates cell-cell contact by promoting high cell migration. The survival rate after single-cell seeding is high, and no apoptosis inhibitors, such as Rho-kinase (ROCK) inhibitor or blebbistatin, are needed. Consequently, the conventional method of maintaining the colony state to prevent apoptosis after re-seeding is unnecessary. Single-cell passaging also reduces the risk of spontaneous differentiation.