Can I culture pluripotent stem cells as single cells on Biolaminin 521 without the use of apoptosis inhibitors?
Yes! Biolaminin 521 successfully recreates the biologically relevant hPSC milieu in vitro, and via integrin binding, Biolaminin 521 induces the PI3K/Akt signaling pathway, promoting high survival and robust long-term self-renewal of human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC). Due to the biologically relevant support from the laminin matrix, pluripotent stem cells can be cultured as single cells without the need for apoptosis inhibitors, such as ROCKi. When transferred from feeder-cells or feeder-free substrates to Biolaminin, the cells might need an adaptation period. It might take up to 5 passages so give it some time. Once the cells are adapted to the Biolaminin 521 matrix, the cells can be cultured as single cells without ROCKi.