Can I re-use the coating solution?

We do not recommend reusing the coating solution, as doing so may affect the consistency and quality of the coating. Instead, we suggest evaluating the optimal coating concentration for your cells and application through titration. Often, a lower concentration than the recommended starting concentration can be used. For Biolaminin 521-adapted hESC or iPSC lines, a coating concentration of 5 µg/mL typically works well. For many MSC lines and neural applications, a coating concentration as low as 1 µg/mL can be effective. The optimal coating concentration is cell type-dependent and should be empirically optimized for specific isoforms and cell lines.