Can I re-use the coating solution?
Due to contamination risks and uncontrollable coating concentration issues, we do not recommend re-using the coating solution but rather to evaluate the optimal coating concentration for your cells and application by titration. Often a lower concentration than the recommended starting concentration can be used. For the culture of Biolaminin 521 adapted hESC or iPSC lines, a coating concentration of 5 µg/mL often works well. For many MSC lines and for many neural applications, a coating concentration as low as 1 µg/mL can often be used. Note that the optimal coating concentration is cell type-dependent and should be optimized empirically for specific isoforms and cell lines.