Can I use Biosilk 521 to generate organoid cultures?

Yes, you can seed your preferred stem cells in, before or after foaming the Biosilk, and then culture them in expansion media until the desired density is reached, before switching to differentiation media.

Depending on your desired 3D culture size, you can use either 96-well or 24-well plates:

Floating 3D cultures:
When using ULA-treated 96-well plates, add 5–10 µL of Biosilk per well to create floating 3D structures. For detailed instructions, see Application Note 012.

Attached 3D cultures:
When using non-treated 24-well plates, add 10–20 µL of Biosilk per well to form attached 3D cultures. Once the cultures reach the desired density, they can be detached from the well surface and optionally cut into smaller pieces for downstream applications. Refer to Instruction For Use 011 for more details.