Can I use Biosilk to generate organoid cultures?

Yes! Biosilk is an excellent 3D culture system for organoid cultures. Seed pluripotent stem cells (or another cell type of interest) either before or after transforming Biosilk 521 into a 3D scaffold by pipetting. Amplify the cells to the desired confluency before switching to your chosen differentiation medium. Once the cells have reached the desired confluency within the Biosilk network, the structure can be cut into smaller pieces (approximately 2 mm thick) using a blade or small scissors and transferred to low-attachment plates for culture as free-floating entities.