Can I use Biosilk to generate organoid cultures?
Yes! It’s an excellent 3D culture system for organoid culture. Seed pluripotent stem cells (or another cell type of interest) in Biosilk-Biolaminin foam attached to the bottom of a cell culture well. Amplify the hPSCs to the desired confluency before switching to a differentiation medium of choice. When cells have reached the desired confluency within the microfibrillar network, manually detach the foam from the bottom of the well using a cell scraper or a pipette tip. Cut the foam structure into 2 -4 pieces (approx. 2 mm thick) using a blade or a pair of small scissors and transfer to new low-attachment culture plates for culture as free-floating entities.