Does the Biolaminin 521 substrate only work with a specific hPSC media?

Biolaminin is compatible with a variety of media. The robust support provided by the Biolaminin 521 culture substrate enables you to work with your preferred medium and enzyme, making it easy to establish fully defined and animal-origin-free protocols. We have successfully tested it with various commercial media, including NutriStem™, mTeSR™1, TeSR™2, and Essential 8™. However, note that cell morphology may vary depending on the medium used for culture.

For feeder-based cultures using AF NutriStem XF, we recommend switching to NutriStem XF/FF when using Biolaminin 521 as the coating substrate.