Does the Biolaminin 521 substrate only work with a specific hPSC media?

The robust support of pluripotent stem cells by the Biolaminin 521 culture substrate allows you to work with your medium and enzyme of choice. As a result, your protocols can easily be made totally defined and animal origin-free. We have successfully tested many different commercial media, such as NutriStem™, mTeSR™1 & TeSR™2, and Essential 8™. However, it is to be expected that cell morphology will look different depending on the medium used for culture.  

If you are using AF Nutristem XF for feeder containing culture, remember to switch to NutriStem XF/FF when using Biolaminin 521 as a coating material.