How do I use Biolaminin for cell culture?

1. Prepare the coating solution:
   Dilute the thawed Biolaminin stock solution with 1x DPBS according to the instructions IN-001.
2. Coat the cultureware:
   Add the diluted Biolaminin solution to tissue culture-treated cultureware. Seal the cultureware (e.g., with Parafilm®) to prevent evaporation.
3. Incubation options:
   • Incubate at +2°C to +8°C overnight.
   • For faster coating, incubate at +37°C for 2 hours.
4. Cell culture:
   Before seeding cells, remove the excess coating solution. No washing is required. Add the cell suspension directly to the Biolaminin-coated surface. For general PSC culture guidelines, see Instructions IN-003.
5. Storage of coated plates:
   If not used immediately, store coated plates and any remaining diluted coating solution at +2°C to +8°C for up to 4 weeks.
   Note: Do not let the coated surface dry, as this will inactivate the laminin coating.

Following these steps ensures an optimal environment for your cell culture applications.