How do I use Biolaminin for cell culture?

Prepare the coating solution:
Dilute the thawed Biolaminin stock solution with 1x DPBS according to Instructions for Use 001.

Coat the cultureware:
Add the diluted Biolaminin solution to tissue culture-treated cultureware. Seal the cultureware (e.g., with Parafilm®) to prevent evaporation.

Incubation options:
Incubate at +2°C to +8°C overnight.
For faster coating, incubate at +37°C for 2 hours.

Cell culture:
Before seeding cells, remove the excess coating solution. No washing is required. Add the cell suspension directly to the Biolaminin-coated surface. For general PSC culture guidelines, refer to Instructions IN-003.

Storage of coated plates:
If not used immediately, store coated plates and any remaining diluted coating solution at +2°C to +8°C for up to 4 weeks.

Note: Do not allow the coated surface dry, as this will inactivate the laminin coating.