How long does it take for the seeded cells to attach to the Biolaminin matrix?
Most cells should have attached within one hour post-seeding and the cells should be evenly distributed over the entire plate. If there’s a lot of cell death after seeding, the cells have most likely been treated too harshly during splitting. After cell attachment, the cell starts to migrate and should have formed small colonies 24h post-seeding. The cells will continue to expand as a homogenous monolayer and are ready to be passaged when cell culture is 60-99% confluent. Depending on the cell line, seeding density, and on the medium used, cultures are usually passaged 3-6 days after seeding.