How long does the laminin coating last in culture? Do I have to add extra laminin to long-term cultures?

Many of our customers successfully use the Biolaminin matrix for long-term culture protocols (lasting several months). The Biolaminin coating remains functional for at least one month in culture. For long-term cultures, if cell detachment is observed, we recommend adding 1-5 µg/mL of additional laminin to the medium (spiking the medium) to improve cell attachment.