How long does the laminin coating last in culture? Do I have to add extra laminin to long-term cultures?

Many of our customers use the Biolaminin matrix for long-term culture protocols (several months). The Biolaminin coating is functional for at least one month in culture. For long-term culture, if cell detachment is noticed, we recommend adding 5 µg/mL extra laminin to the medium (spiking the medium) to improve attachment.