Is it necessary to use DPBS Ca++/Mg++ to dilute Biolaminin stock solution?

Ca²⁺ and Mg²⁺ are divalent cations important for maintaining protein structure and function. When diluting Biolaminins, using DPBS with Ca²⁺/Mg²⁺ is preferable. However, based on our in-house experience, Biolaminins are stable proteins and can also be diluted in DPBS without Ca²⁺/Mg²⁺. The absence of these cations does not affect coating efficiency or protein functionality.