Is it possible to measure the coating efficiency of the Biolaminin substrates?

In-house, we typically use functional cell attachment to estimate coating optimization. By seeding the same number of cells onto plates coated with different amounts of Biolaminin and measuring cell confluence the next day, we can identify the optimized coating concentration for the tested cell line. The optimal coating concentration is reached when most cells attach, and the monitored confluence (%) no longer increases with higher coating concentrations. The optimal concentration may vary between cell lines, but we generally recommend starting with 10 µg/mL and decreasing to 5 µg/mL once the cells have adapted to the Biolaminin culture conditions.

Another method to assess coating efficiency is ELISA. One of our customers used an ELISA method where they coated plates with varying amounts of Biolaminin and employed an antibody (Abcam ab11575, rabbit polyclonal against mouse EHS) to detect and measure the laminin protein. This antibody recognizes various laminin chains and can be used for this general purpose. If different laminin isoforms are used for coating, monoclonal antibodies specific to each laminin chain are available from Atlas Antibodies (Laminins Marker Panel), allowing for the separation and identification of individual isoforms.