Is it possible to measure the coating efficiency of the Biolaminin substrates?

In-house, we only use cell attachment to estimate coating optimization (not a quantitative method). By seeding the same numbers of cells on the plate that is coated with different amounts of Biolaminin and read the cell confluence next day post-seeding, we can see the optimized coating concentration of the tested cell line. When most cells attach and when the monitored confluence (%) do not increase along with the increased coating concentration the optimal coating concentration has been reached. The optimized coating concentration can be different from cell line to cell line, and we generally recommend using 10 µg/ml as a starting concentration, decreasing the coating concentration to 5 µg/ml once the cells have adapted to the Biolaminin culture conditions.

Another method would be ELISA. A customer of ours has tested an ELISA method where they coated the plate with different amounts of Biolaminin and used an antibody (Abcam ab11575, rabbit polyclonal against mouse EHS) to detect and measure the laminin protein. This antibody recognizes different kinds of laminin chains and could be commonly used for this purpose. However, if more than one type of laminin is used for coating, there are monoclonal antibodies to each laminin chain available from Atlas antibodies.