What are the major advantages of using human recombinant, full-length laminins vs. truncated or tissue-originated laminin products?

Our Biolaminin cell culture matrices are the only full-length, recombinant laminins on the market, with all the functional domains intact.

Laminins isolated from tissues are an impure mix of several ECM proteins. Moreover, the proteins are heavily degraded during isolation and lose structural integrity and function. A fractionated or truncated laminin molecule, or undefined laminins isolated from tissues, lacks many of the domains that are needed by the cells to form a proper extracellular matrix network and to stimulate cellular signal transductions. Hence, only the intact, recombinant full-length laminin can create a more authentic and defined cell culture environment.

For reference about the difference in quality and function of commercially available recombinant full laminins versus isolated preparations of laminins, see Wondimu et al., 2006.

Biolaminin substrates are always full laminin proteins.