What is the recommended Biolaminin coating concentration?

For culturing hESC, iPSC lines, and MSCs, a starting Biolaminin 521 concentration of 10 µg/mL is recommended. Once the cells are adapted to the Biolaminin 521 coating, the concentration can generally be reduced to 5 µg/mL. The optimal coating concentration is cell type-dependent and should be optimized empirically for specific isoforms and cell lines. For certain specialized cells, such as cardiomyocytes, a higher coating concentration (10 µg/mL) may be required. Using too low a coating concentration could lead to slow growth or uneven cell spreading.