What is the recommended Biolaminin coating concentration?

For the culture of hESC or iPSC lines, 10 µg/mL Biolaminin 521 is recommended as a starting concentration. Once the cells have been adapted to the Biolaminin 521 coating, the coating concentration generally can be lowered to 5 µg/mL. The optimal coating concentration is cell type-dependent and should be optimized empirically for specific isoforms and cell lines. For many MSC lines and for many neural applications, a coating concentration as low as 1 µg/mL can often be used. For the culture of some specialized cells, such as cardiomyocytes, a higher coating concentration (10 µg/mL) might be required. A too low coating concentration could result in slow growth or an uneven cell spread.