What is the smallest volume I can use and still get a good foam?

For 96-well plates, a final volume as low as 5 µL, including cells, is feasible. Ensure that the Biosilk solution is not diluted below 2 mg/mL. For foaming, set the pipette to twice the volume of the Biosilk solution (e.g., 10 µL for 5 µL of foam). For further details, please refer to the 96-well plate guide.