When thawing hPSCs grown on Biolaminin, what is the recovery like?

The post-thaw cell survival is high when supported by Biolaminin 521, especially when frozen and thawed as single cells. This is nicely described in a publication by Miyazaki and colleagues, where they show that hPSC that were frozen and thawed as colonies showed markedly decreased survival compared to cells frozen and thawed as single cells where the majority of the cells were viable (Miyazaki et al., 2013).