Which enzymes do you recommend for passaging PSCs growing on Biolaminin?

You can use a dissociation reagent of choice (e.g. TrypLE select, Trypsin-EDTA, EDTA, Accutase). The incubation time depends on the solution used to dissociate the cells and the specific cell line. Please note that cells often attach tighter on Biolaminin compared to other matrices and scraping or pipetting without first loosing up cells can affect cell integrity and viability which could result in less attachment the next day. Less confluent cells need shorter treatment time whereas more confluent cells might need longer treatment time. The cells should detach easily without too much pipetting needed. Stem cells are sensitive, and too long exposure to dissociation enzymes or too much mechanical force applied may result in lower cell viability.