Which range of seeding density do you recommend for human pluripotent stem cells?
Optimal seeding densities can vary depending on the specific cell line and should be determined empirically for your system. Biolaminin 521 has been shown to support cell survival at densities as low as 5,000 cells/cm². However, we generally recommend seeding at a density of 30,000–50,000 cells/cm² or splitting your cells at a ratio of 1:10 to 1:30 (Rodin et al., 2014). Biolaminin 521 also supports single-cell expansion, such as during gene editing. For detailed guidance, please refer to our application notes:
Application Note 004: Expansion and maintenance of pluripotent stem cells