Which range of seeding density do you recommend for human pluripotent stem cells?

Optimal seeding densities will vary from one cell line to another and should be determined empirically for your system. Biolaminin 521 has been shown to support cell survival of as low as 5,000 cells/cm2. However, we generally recommend seeding your cells at a concentration of 30 000-50 000 cells/cm2 or split your cells with a ratio of 1:10 to 1:30.