Why does the foam break into pieces (disperse) when I add the medium?

The foam may break into pieces for one of these reasons:

1. Extended time at RT: If the Biosilk solution has been left at room temperature too long after thawing (more than 1 hour), the protein may partially precipitate, causing a milky appearance. The solution should be used immediately after thawing.
2. Improper stabilization process: Ensure the stabilization process is carried out according to the protocol at 37°C to maintain foam integrity.
3. Abrupt medium addition: Adding medium with excessive force can disrupt or lift the foam. Carefully drop the medium around and then on top of the stabilized foam to avoid breaking it.
4. Excessive dilution of Biosilk: The final concentration of Biosilk should not be lower than 2 mg/mL. If adding another Biolaminin isoform to the Biosilk, ensure the total added Biolaminin volume does not exceed 1/10 of the Biosilk volume.
5. Improper pipetting: When creating the foam, pipet 15-25 times rapidly within 10-15 seconds to generate small, evenly distributed bubbles. Avoid creating overly large bubbles, as they can create holes. Once foaming is complete, do not continue pipetting, as this can disrupt the 3D structure.