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Can I do immunocytochemistry of the cells in Biosilk?
Yes! Fix the silk/cell construct in 4% paraformaldehyde, embed them (e.g. Tissue-Tek), and section (e.g. using a Leica cryostat). The procedure is […]
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Can you extract cells from the Biosilk foam, without any residual spider silk being present in the final cell suspension?
Yes, it is possible to enzymatically release (e.g. trypsin, Accutase) cells from the silk form of Biosilk. In the silk […]
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Would Biosilk only be applicable for soft tissue organoid models? What about bone models?
It is possible to integrate Biosilk into ceramic material and during biomimetic mineralization of calcium phosphate (see upcoming article).