Biolaminin 121 LN (LN121)
Human recombinant laminin 121
Biolaminin 121 is a full-length and animal-origin free human laminin 121 protein, that can be used as a general attachment protein for most cell types in vitro.
A defined culture substrate for many cell types
Laminins 111 and 121 are widely expressed during embryogenesis and are important for early epithelial development and differentiation of the epiblast. Laminins induce different cell signaling cascades and co-signal with various growth factors in a cell-type-dependent manner. Biolaminin 121 LN (LN121-02 and LN121-0501) can support the survival, proliferation, and differentiation of many different cell types in vitro.
Improved neurite outgrowth and postsynaptic myotubes
Laminin 121 has a higher affinity to integrin receptors compared to laminin-111, especially to integrins α7β1 and α6β1 (Sasaki, 2010). Laminin 121 has been shown to be especially supportive to neurite outgrowth (Sasaki, 2010). Human myotubes cultured on a combination of Biolaminin-121 and -221 were also shown to have the most developed clusters of postsynaptic machinery (Pęziński, 2020).
Skeletal muscle cellsBiorelevant culture of muscle cells on Biolaminin substrates Laminin 211, 221 and 521 form an important part of the adult skeletal muscle microenvironment Laminin […]VIEW application
Bone and cartilage cellsBiorelevant culture of bone and cartilage cells on Biolaminin substrates High expression of laminin isoforms in the bone microenvironment Bone cells, including include […]VIEW application
Mammary gland cellsBiorelevant culture of human mammary cells on Biolaminin substrates Laminins have both overlapping and unique functions in the mammary gland tissue Mammary […]VIEW application
Lung cellsBiorelevant culture of lung cells on Biolaminin substrates Laminin expression in lung Laminin is key proteins in the basement membranes of the airway […]VIEW application
Intestinal cellsBiorelevant culture of intestinal cells on Biolaminin substrates Laminin expression in the intestine The small intestine contains mucosal epithelial invaginations called […]VIEW application
Hepatocytes and hepatoblastsBiorelevant culture of liver cells on Biolaminin substrates Several laminins play a vital role in liver progenitor cell-mediated regeneration The […]VIEW application
1. Slowly thaw recombinant laminins at +4°C before use.
2. Dilute the thawed laminin stock solution with 1xDPBS containing Ca2+ and Mg2+.
3. Add the diluted laminin solution to tissue culture-treated cultureware for a final coating concentration of 0.5-2 μg/cm2. The optimal coating concentration is cell line-dependent.
4. Seal the plate (e.g. with Parafilm®) to prevent evaporation and incubate at +2°C to +8°C overnight. If a more rapid coating is required, incubate at +37°C for 2 hours. Make sure the laminin solution is spread evenly across the surface. Note that the laminin matrix will be inactivated if let dry.
- The laminin stock solution is long-term stable when stored at -20°C to -80°C. Please refer to the product-specific CoA for shelf life details.
- Repeated freeze-thawing should be avoided. If desired, the laminin stock can be dispensed into working aliquots and stored at -20°C to -80°C. Thawed, undiluted laminin stock is stable for at least 3 months when stored at +2°C to +8°C under aseptic conditions.
- Avoid long exposure of the protein to ambient temperatures.
- For your convenience, the coated plates can be kept for up to 4 weeks when stored aseptically at +2°C to +8°C.
- The protocol can easily be made totally defined and animal origin-free with your choice of culture medium and enzyme.
- Before start, all solutions used for cell passaging should be aliquoted in sufficient amounts and pre-warmed at +37°C, 5% CO2.
Biolaminin 121 LN
For research use only
-20°C to -80°C
Clear, colorless, buffered solution with a
pH of 7.2 with 10% glycerol and 0.02% NaN3
Laminin 121 (Laminin-3)
Animal origin-free, human recombinant protein
General cell culture substrate. Can be found in the placenta, skeletal muscles, and kidneys.
Laminin 121 – recombinant expression and interactions with integrins
Sasaki T., Takagi J., Giudici C., Yamada Y., Arikawa-Hirasawa E., Deutzmann R., Timpl R., Sonnenberg A., Bächinger H.P., Tongeh D.
Matrix Biol., 2010
An improved method for culturing myotubes on laminins for the robust clustering of postsynaptic machinery
Marcin Pęziński, Patrycja Daszczuk, Bhola Shankar Pradhan, Hanns Lochmüller, Tomasz J Prószyński
Scientific Reports, 2020
Not sure how much laminin you need? To make it easy, we have created a tool where you can calculate the amount needed for your experiments. Just choose culture well format and fill in the desired coating concentration to see the amount required.
Please see the coating instructions for concentration and volume guidelines.