Biolaminin 121 LN (LN121)
Human recombinant laminin 121
Biolaminin 121 is a full-length and animal-origin free human laminin 121 protein, that can be used as a general attachment protein for most cell types in vitro.
A defined culture substrate for many cell types
Laminins 111 and 121 are widely expressed during embryogenesis and are important for early epithelial development and differentiation of the epiblast. Laminins induce different cell signaling cascades and co-signal with various growth factors in a cell-type-dependent manner. Biolaminin 121 LN (LN121-02 and LN121-0501) can support the survival, proliferation, and differentiation of many different cell types in vitro.
Improved neurite outgrowth and postsynaptic myotubes
Laminin 121 has a higher affinity to integrin receptors compared to laminin-111, especially to integrins α7β1 and α6β1 (Sasaki, 2010). Laminin 121 has been shown to be especially supportive to neurite outgrowth (Sasaki, 2010). Human myotubes cultured on a combination of Biolaminin-121 and -221 were also shown to have the most developed clusters of postsynaptic machinery (Pęziński, 2020).
Skeletal muscle cellsBiorelevant culture of muscle cells on Biolaminin substrates Laminin 211, 221 and 521 form an important part of the adult skeletal muscle microenvironment Laminin […]View application
Bone and cartilage cellsBiorelevant culture of bone and cartilage cells on Biolaminin substrates High expression of laminin isoforms in the bone microenvironment Bone cells, including include […]View application
Mammary gland cellsBiorelevant culture of human mammary cells on Biolaminin substrates Laminins have both overlapping and unique functions in the mammary gland tissue Mammary […]View application
Lung cellsBiorelevant culture of lung cells on Biolaminin substrates Laminin expression in lung Laminin is key proteins in the basement membranes of the airway […]View application
Intestinal cellsBiorelevant culture of intestinal cells on Biolaminin substrates Laminin expression in the intestine The small intestine contains mucosal epithelial invaginations called […]View application
Hepatocytes and hepatoblastsBiorelevant culture of liver cells on Biolaminin substrates Several laminins play a vital role in liver progenitor cell-mediated regeneration The […]View application
Sasaki T., Takagi J., Giudici C., Yamada Y., Arikawa-Hirasawa E., Deutzmann R., Timpl R., Sonnenberg A., Bächinger H.P., Tongeh D.
Matrix Biol., 2010
An improved method for culturing myotubes on laminins for the robust clustering of postsynaptic machinery
Marcin Pęziński, Patrycja Daszczuk, Bhola Shankar Pradhan, Hanns Lochmüller, Tomasz J Prószyński
Scientific Reports, 2020
Not sure how much laminin you need? To make it easy, we have created a tool where you can calculate the amount needed for your experiments. Just choose culture well format and fill in the desired coating concentration to see the amount required.
Please see the coating instructions for concentration and volume guidelines.