Biolaminin 121 LN (LN121)

Human recombinant laminin 121

Biolaminin 121 is a full-length and animal-origin free human laminin 121 protein, that can be used as a general attachment protein for most cell types in vitro – in particular for hepatic and neural differentiation.

A defined culture environment

Laminins 111 and 121 are widely expressed during embryogenesis, and are important for early epithelial development and differentiation of the epiblast.

Laminin-121 is one of the α1 chains containing laminins, the other isoform being laminin-111. In the adult, laminin α1 chain expression is relatively restricted and is only present in some epithelial basement membranes. It is primarily found in tissues such as the eye, liver, and kidney, whereas hardly at all expressed in endothelial, adipose, nervous, or muscle tissues (Ekblom, 2003; Virtanen, 2000).

Biolaminin 121 LN (LN121-02 and LN121-0501) can support the survival, proliferation, and differentiation of many different cell types in vitro. LN121 improves attachment and proliferation of most cell types and are particularly useful for hepatic and neural differentiation. Biolaminin 121 also effectively supports neurite outgrowth. Laminin-121 has a higher affinity to integrin receptors compared to laminin-111 with the highest affinity to α6β1 and α7β1 integrins (Sasaki, 2010).

In addition to inducing different cell signaling cascades, laminin-121 is known to co-signal with various growth factors in a cell type dependent manner. This may be an explanation for the major biological effects that laminin 121 exerts on a wide selection of cell types.

  • Neural cells

    Biorelevant culture of neural cells on Biolaminin substrates Laminins are widely expressed in the nervous system and are essential for […]
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  • Bone and cartilage cells

    Biorelevant culture of bone and cartilage cells on Biolaminin substrates High expression of laminin isoforms in the bone microenvironment Bone cells, including include […]
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  • Mammary gland cells

    Biorelevant culture of human mammary cells on Biolaminin substrates Laminins have both overlapping and unique functions in the mammary gland tissue Mammary […]
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  • Lung cells

    Biorelevant culture of lung cells on Biolaminin substrates Laminin expression in lung Laminin is key proteins in the basement membranes of the airway […]
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  • Intestinal cells

    Biorelevant culture of intestinal cells on Biolaminin substrates Laminin expression in the intestine The small intestine contains mucosal epithelial invaginations called […]
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  • Hepatocytes and hepatoblasts

    Biorelevant culture of hepatocytes on Biolaminin substrates Laminins play a vital role in liver progenitor cell-mediated regeneration The liver contains […]
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Laminin 121 – recombinant expression and interactions with integrins

Sasaki T., Takagi J., Giudici C., Yamada Y., Arikawa-Hirasawa E., Deutzmann R., Timpl R., Sonnenberg A., Bächinger H.P., Tongeh D.

Matrix Biol., 2010

Size guide

Not sure how much laminin you need? To make it easy, we have created a tool where you can calculate the amount needed for your experiments. Just choose culture well format and fill in the desired coating concentration to see the amount required.

Please see the coating instructions for concentration and volume guidelines.