Biolaminin 332 LN (LN332)

Human recombinant laminin 332

Biolaminin 332 is a full-length and animal-origin free human laminin 332 product, which supports cells in epithelial basement membranes in several tissues.

A biologically relevant culture environment

Laminin 332 highly influences keratinocyte proliferation and mediates a strong adhesion of keratinocytes to the basement membrane through hemidesmosome protein complex (Ghohestani, 2001). Laminin 332 is highly enriched in epithelial basement membranes.

The importance of laminin 332 mediated adhesion in the skin is also evident in the human blistering disease Junctional Epidermolysis Bullosa, which is associated with laminin-332 gene mutations (DiPersio, 1997).

The expression of laminin 332 is upregulated during wound healing and has important effects on keratinocyte migration, invasion and eventually tissue remodeling (D’Alessio, 2008).

Laminin 332 is highly expressed in the catagen phase by keratinocytes in the basement membrane zone of hair follicles. It antagonizes laminin 511 driven hair growth in vitro of human hair follicles and, thus, hair growth has been suggested to be controlled by the relative expression of laminin 511 and laminin 332 (Conti, 2003; Sugawara, 2007; Li, 2003).

Structural changes in the RPE basement/Bruch’s membrane (BrM) lead to alterations in the RPE-matrix adhesion which is commonly the cause for retinal diseases (Zarbin, 2004).

Laminins are produced by and secreted from RPE cells themselves into the RPE basement membrane (Campochiaro, 1986). In particular, the RPE cells produce and bind the strongest to laminin 332 and in vitro, laminin 332 preserves phenotypic stability of mature RPE cells the best (Aisenbrey, 2006).

  • Cancer cells

    Biorelevant culture of cancer stem cells on Biolaminin substrates Cancer cells use laminin/integrin signaling pathways that promote tumor cell growth, invasion, and […]
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  • Bone and cartilage cells

    Biorelevant culture of bone and cartilage cells on Biolaminin substrates High expression of laminin isoforms in the bone microenvironment Bone cells, including include […]
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  • Skin cells

    Biorelevant culture of keratinocytes on Biolaminin substrates The basement membrane composition in the epidermis Human epidermal keratinocyte (HEK) cells are […]
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  • Hair follicle cells

    Biorelevant culture of hair follicle stem cells on Biolaminin substrates Epithelial tissues contain a-5 and a-3 laminin isoforms Epithelial tissues are […]
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  • Mesenchymal stem cells

    Biorelevant culture of human mesenchymal stem cells on Biolaminin substrates Laminin 521 is widely expressed in the bone marrow and is an […]
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Size guide

Not sure how much laminin you need? To make it easy, we have created a tool where you can calculate the amount needed for your experiments. Just choose culture well format and fill in the desired coating concentration to see the amount required.

Please see the coating instructions for concentration and volume guidelines.