Biolaminin 511 LN (LN511)
Human recombinant laminin 511
Biolaminin 511 is the natural laminin for mouse embryonic stem cells and allows sustained pluripotency without the need to use feeder cells or differentiation inhibitors like LIF.
Biorelevant microenvironment keeps mouse stem cells pluripotent for months
The need for matrices that support pluripotent stem cell renewal in a reproducible manner is clear. Stem cell biologists have long experienced problems with the repeatability of results and with the maintenance of pluripotency when working with ES cell cultures.
Laminin 511, together with laminin 521, are the most widely expressed laminins in the body. Stem cells grow as monolayers on LN511 enabling equal contact to both the matrix and cell culture medium, which leads to a more homogenous cell population. Since Biolaminin 511 is a recombinant protein, lot-to-lot variation is minimal and therefore fewer replicates are needed for reliable results (Domogatskaya, 2008; Rodin, 2010).
Mouse ES an LN511 matrix creates a biological niche keeping the ES cells in a pluripotent state for months. Biolaminin 511 is a full-length human recombinant laminin that is used as a mouse ESC or iPSC culture substrate.
LN511 is especially recommended for the cell culture of murine embryonic and induced pluripotent stem cells, while LN521 is recommended for the culture of human stem cells.
Biolaminin 511 supports human epithelial cell culture
Laminin 511 is an important regulator of hair development and an adhesion molecule for human epidermal keratinocytes supporting cell survival and growth (Yap, 2019). Laminin 511 is one of the laminins present in Bruch’s membrane located beneath the retinal pigment epithelium in the eye. Full-length laminin 511 has been shown to allow ex vivo expansion of human limbal epithelial cells of the cornea (Shibata, 2018). Precoating with Biolaminin 511 was also shown to promote the functional recovery of transplanted corneal endothelial cells (CECs) (Zhao, 2020).
Biolaminin 511 allows robust and easy long-term expansion of primary adult human dermal keratinocytes in a defined and animal-origin free manner (Tjin, 2018). Keratinocyte culture was comparable to the 3T3-J2 feeder co-culture system, in terms of colony-forming efficiency, basal marker profile, genetic stability and the ability to form normal stratified epidermal structure in both in vitro and in vivo models. Keratinocyte cells cultivated on Biolaminin 511 also demonstrate a significantly reduced pro-inflammatory signalling pathway expressions compared to classical feeder culture-based systems.
Organotypic culture shows that human keratinocytes cultured on Biolaminin 511 are able to form a normal stratified epidermal structure in vitro, forming a normal epidermal skin layer with stratum corneum, stratum granulosum, stratum spongiosum, and stratum basal similar to that of a normal skin. (Tjin 2020, Tjin 2018)
Stem cells of animal originBiorelevant culture of animal stem cells on Biolaminin substrates The laminin protein family is highly conserved Laminins are a large family of […]VIEW application
Skin cellsBiorelevant culture of keratinocytes on Biolaminin substrates The basement membrane composition in the epidermis Human epidermal keratinocyte (HEK) cells are […]VIEW application
Hair follicle cellsBiorelevant culture of hair follicle stem cells on Biolaminin substrates Epithelial tissues contain a-5 and a-3 laminin isoforms Epithelial tissues are […]VIEW application
Intestinal cellsBiorelevant culture of intestinal cells on Biolaminin substrates Laminin expression in the intestine The small intestine contains mucosal epithelial invaginations called […]VIEW application
Mammary gland cellsBiorelevant culture of human mammary cells on Biolaminin substrates Laminins have both overlapping and unique functions in the mammary gland tissue Mammary […]VIEW application
Eye cellsBiorelevant culture of eye cells Laminin proteins are important components in the eye microenvironment Biolaminin products have been succesfully used […]VIEW application
Rodin S, domogatskaya A, Ström S, Hansson EM, Chien KR, Inzunza J, Hovatta O, Tryggvason K.
Nature Biotechnology, 2010
Compositional and structural requirements for laminin and basement membranes during mouse embryo implantation and gastrulation
Miner JH, Li C, Mudd JL, Go G, Sutherland AE.
Trophoblast-specific expression and function of the integrin alpha 7 subunit in the peri-implantation mouse embryo
Klaffky E,Williams R,Yao CC, Ziober B, Kramer R, Sutherland AE.
Developmental Biology, 2001
Not sure how much laminin you need? To make it easy, we have created a tool where you can calculate the amount needed for your experiments. Just choose culture well format and fill in the desired coating concentration to see the amount required.
Please see the coating instructions for concentration and volume guidelines.