Biolaminin 521 CTG (CT521)

Human recombinant laminin 521

Biolaminin® 521 CTG is a full-length, human, recombinant laminin-521 substrate, the only one of its kind on the market, providing an optimal environment for feeder-free culture of human PSCs, MSCs and most anchorage-dependent progenitor cell types. With this clinical-grade product, scientists are supported throughout their cell therapy development process – from concept to commercialized therapy.

The only full-length laminin proteins on the market

Biolaminin 521 CT (cell therapy grade; CT521-02 and CT521-05) comprises full-length, human recombinant laminin 521 protein, a cell culture matrix designed for robust cultivation of human pluripotent stem cells (hPSCs) for clinical applications. As an inherent stem cell niche protein, laminin-521 provides a biologically relevant environment leading to improved survival and efficient growth from single-cell cultures to large-scale production.

Reproducible stem cell culture process

By replicating the native hPSC microenvironment, the Biolaminin 521 substrate facilitates optimal attachment, high survival rates, and sustained self-renewal of pluripotent stem cells in vitro.

Laminin-521 standardizes pluripotent gene expression profiles, maximizes genetic stability, and activates pertinent cell signaling pathways, ensuring uniform cellular responses and improved functionality.

Biolaminin 521 products in different grades – from research to clinical use.

Controlled differentiation with tissue-specific laminin

Beyond its fundamental role in sustaining stem cell cultures, laminin-521 also augments cell differentiation, fostering the maturation, polarization, and organization of specialized cell types. It stands as the preferred option for culturing a set of primary cell types given its natural occurrence in tissues such as the nervous system, pancreas, and eye.

From research to clinical use

Focused on clinical applications? The Biolaminin MX and CT laminin-521 products are designed for translation with extensive regulatory documentation.

Whatever your research aim is, you need a culture substrate that guarantees the highest cell quality and yield. Read more about the related cell applications.

  • Clinical applications and large-scale cell production

    Biolaminin substrates for large-scale manufacturing and clinical use Scaling up high-quality cell production for clinical needs A scalable system for […]
    View application
  • Pluripotent stem cells

    Biorelevant culture of human ES and iPS cells on Biolaminin substrates Biolaminin 521 successfully replicates the genuine human stem cell niche […]
    View application
  • 3D culture

    Biosilk and Biolaminin key advantages 3D cell culture and organoid formation 3D culture systems for disease modeling, drug screening, and […]
    View application
  • Clonal stem cell culture

    Biorelevant clonal stem cell culture on Biolaminin substrates Biolaminin 521 successfully replicates the genuine human stem cell niche in vitro […]
    View application
  • iPSC reprogramming and expansion

    Reprogramming and expansion of human iPS cells on Biolaminin substrates High-efficiency iPSC reprogramming and expansion on Biolaminin 521 Human recombinant Biolaminin […]
    View application
  • Neural cells

    Biorelevant culture of neural cells on Biolaminin substrates Laminins are widely expressed in the nervous system and are essential for […]
    View application
  • Hepatocytes and hepatoblasts

    Biorelevant culture of liver cells on Biolaminin substrates Several laminins play a vital role in liver progenitor cell-mediated regeneration The […]
    View application
  • Eye cells

    Biorelevant culture of eye cells Laminin proteins are important components in the eye microenvironment Biolaminin products have been succesfully used […]
    View application
  • Endothelial cells

    Biorelevant culture of endothelial cells Biolaminin isoforms for vascular endothelial cell culture Endothelial cells that make up the vascular network […]
    View application

Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment

Rodin S., Antonsson L., Niaudet C., Simonson O.E., Salmela E., Hansson E.M., Domogatskaya A., Xiao Z., Damdimopoulou P., Sheikhi M., Inzunza J., Nilsson A.S., Baker D., Kuiper R., Sun Y., Blennow E., Nordenskjöld M., Grinnemo K.H., Kere J., Betsholtz C., Hovatta O., Tryggvason K.

Nature Communications, 2014

Monolayer culturing and cloning of human pluripotent stem cells on laminin-521 based matrices under xeno-free and chemically defined conditions

Rodin S., Antonsson L., Hovatta O., Tryggvason K.

Nature Protocols, 2014

a-5 Laminin Synthesized by Human Pluripotent Stem Cells Promotes Self-Renewal

Laperle A., Hsiao C., Lampe M., Mortier J., Saha K., Palecek S.P., and Masters K.S.

Stem Cell Reports, 2015

Laminin 521 stabilizes the pluripotency expression pattern of human embryonic stem cells initially derived on feeder cells

Albalushi H., Kurek M., Karlsson L., Landreh L., Rós Kjartansdóttir K., Söder O., Hovatta O., Stukenborg J-B.

Stem Cell International, 2017

Niche-derived laminin-511 promotes midbrain dopaminergic neuron survival and differentiation through YAP

Zhang D., Yang S., Toledo E.M., Gyllborg D., Saltó C., Villaescusa J.C., Arenas E.

Sci Signal. 2017

Size guide

Not sure how much laminin you need? To make it easy, we have created a tool where you can calculate the amount needed for your experiments. Just choose culture well format and fill in the desired coating concentration to see the amount required.