Biosilk™ is a 3D culture matrix for the expansion and long-term differentiation of human primary cells and a scaffold for organoid formation.

Benefits of Biosilk for the 3D culture of stem cells and organoids

In the Biosilk fibrillar network, oxygen, nutrients, and patterning factors can flow into the inner part of the organoid. Cells are not encapsulated.This enables long-term differentiation protocols and makes it possible to generate larger organoids with uniform cellular specialization and organization, without an increased risk of getting necrotic centers.

Biosilk is a completely defined recombinant spider silk protein matrix that is non-immunogenic and biodegradable, further facilitating its use in clinical applications.

All our matrices are chemically defined and animal origin-free, which makes them ideal substrates for each level of the scientific process – from basic research to clinical applications.

Biosilk can self-assemble into a microfibrous network, such as foams. Due to its favorable functional and mechanical properties, Biosilk can support extensive cellular remodeling, self-organization, and morphogenesis.

3D models are pursued to reproduce the way cells grow and interact with each other and the microenvironment in native tissues. To provide proper microenvironmental contacts and support correct cell phenotype, Biolaminin isoforms can be added into different organoid culture systems. Especially, the Biosilk 3D network can be easily biofunctionalized with Biolaminin isoforms and other extracellular matrix proteins. This results in a more tissue-like microenvironment in which the integrin-involved attachment to the Biosilk fibers gives the cells an elongated shape, with organized cytoskeletons and the formation of defined focal adhesion points.Increased biological relevance increases the chances of capturing robust and applicable experimental and pre-clinical results.

Biosilk is made of recombinant silk protein

Biosilk fibers consist of short strings of spider silk protein, spidroins, which are biofunctionalized with an RGD-domain sequence. Biosilk can be further supplemented with any of the Biolaminin substrates for improved cell functionality.

Simple to use – no special equipment needed

This video describes how to generate Biosilk 3D foam scaffolds for the expansion and differentiation of human pluripotent stem cells.

Homogenous organoids without necrotic centers

Ventral midbrain organoids with Biosilk and Biolaminin 111 (below). Adapted from Fiorenzano et al. 2021 Nature Communications Fig 7. (CCBY4.0).

  • 3D culture

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  • Pluripotent stem cells

    Biorelevant culture of human ES and iPS cells on Biolaminin substrates Biolaminin 521 successfully replicates the genuine human stem cell niche […]
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  • Intestinal cells

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  • Pancreatic cells

    Biorelevant culture of pancreatic cells on Biolaminin substrates The basement membrane formed next to the beta-cells contains alpha-4 and alpha-5 laminin […]
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  • Hepatocytes and hepatoblasts

    Biorelevant culture of liver cells on Biolaminin substrates Several laminins play a vital role in liver progenitor cell-mediated regeneration The […]
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  • Lung cells

    Biorelevant culture of lung cells on Biolaminin substrates Laminin expression in lung Laminin is key proteins in the basement membranes of the airway […]
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  • Eye cells

    Biorelevant culture of eye cells Laminin proteins are important components in the eye microenvironment Biolaminin products have been succesfully used […]
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Assembly of functionalized silk together with cells to obtain proliferative 3D cultures integrated in a network of ECM-like microfibers

Johansson U, Widhe M, Shalaly ND, Arregui IL, Nilebäck L, Tasiopoulos CP, Åstrand C, Berggren PO, Gasser C, Hedhammar M.

Scientific reports, 2019

Biological responses to spider silk-antibiotic fusion protein

Gomes S, Gallego-Llamas J, Leonor IB, Mano JF, Reis RL, Kaplan DL.

J Tissue Eng Regen Med, 2017