A Scalable Suspension Platform for Generating High-Density Cultures of Universal Red Blood Cells from Human Induced Pluripotent Stem Cells

This article describes a scalable suspension agitation culture platform for the differentiation of human induced pluripotent stem cell-microcarrier aggregates into functional red blood cells. The report describes the cell quantity and quality in culture sizes ranging from 6-well plates to 500 ml spinner flasks, reaching up to 17 million high-quality cells per ml. The process could find applications in future large-scale red blood cell production in controlled bioreactors.