Agitation conditions for the culture and detachment of hMSCs from microcarriers in multiple bioreactor platforms
Alvin W. Nienow, Christopher J. Hewitt, Thomas R.J. Heathman, Veronica A.M. Glyn, Gonҫalo N. Fonte, Mariana P. Hanga, Karen Coopman, Qasim A. Rafiq.
Biochemical Engineering Journal, 2016
In this article, 22 combinations of conditions in which hMSCs have been cultivated on microcarriers are reported – four different types and sizes of bioreactor. Biolaminin-521 surface coating was applied in two of them. The agitation strategy described is suggested to offer a basis for scale-up.
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