Animal Substance-Free Human Embryonic Stem Cells Aiming at Clinical Applications
Hovatta O., Rodin S., Antonsson L., and Tryggvason K.
Stem Cells Transl Med, 2014
In this concise review, the authors describe that it is now feasible to establish clinical-grade hESC lines in animal substance-free chemically defined conditions with the Laminin-521 matrix. They discuss recent developments and the progress to now being able to generate genetically stable hESC lines even from single biopsied blastomeres without destroying the embryo.
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