Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells

Wang Y., Alhaque S., Cameron K., Meseguer-Ripolles J., Lucendo-Villarin B., Rashidi H., Hay D.C.

JoVE, 2017

Here, the authors show in a video how they developed a defined optimized system for hepatocyte differentiation using human recombinant laminins as extracellular matrices in combination with a serum-free differentiation process. The protocol shows the procedures for culturing hPSCs on LN521 and differentiating them on either LN521 or a blend of LN521 and LN111 (Biolaminin 521 and 111). Highly efficient hepatocyte specification was achieved, with demonstrated improvements in both HLC function and phenotype. Importantly, this system is easy to scale up using research and GMP-grade hPSC lines promising advances in cell-based modeling and therapies.