Differentiation-dependent Expression of Laminin-8 (α4β1γ1) mRNAs in Mouse 3T3-L1 Adipocytes

Here, the authors report that laminin-411 is the specific isoform of laminin synthesized in adipocytes. Reverse transcription-polymerase chain reaction (RT-PCR) of mRNA from mouse 3T3-L1 cells yielded amplified fragments only for α4β1γ1. Northern blots showed that the levels of α4,β1, and γ1 mRNAs increased 2.5-fold during adipose conversion of 3T3-L1 cells. A 1062 bp cDNA fragment cloned by RT-PCR demonstrated a polymorphism in the mouse a4 gene which would lead to five amino acid changes in the domain G.