Dynamic Heterogeneity and DNA Methylation in Embryonic Stem Cells
Singer Z.S., Yong J., Tischler J., Hackett J.A., Altinok A., Surani M.A., Cai L., and Elowitz M.B.Cell Press, 2014
Analyzis of gene expression in individual ESCs using single-molecule RNA-FISH and quantitative time-lapse movies. Fluorescence Time-Lapse Microscopy and Data analysis on reporter cells mixed with unlabeled parental cells at 1:9 ratio and plated at a total density of 20,000 cells/cm2 on glass-bottom plates (MatTek) coated with human laminin-511 >12 hr before imaging.
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