Efficiently Specified Ventral Midbrain Dopamine Neurons from Human Pluripotent Stem Cells Under Xeno-Free Conditions Restore Motor Deficits in Parkinsonian Rodents
Jonathan C Niclis, Carlos W Gantner, Walaa F Alsanie, Stuart J McDougall, Chris R Bye, Andrew G Elefanty, Edouard G Stanley, John M Haynes, Colin W Pouton, Lachlan H Thompson, Clare L Parish
Stem Cells Transl Med, 2017
The authors describe the first fully defined feeder- and xenogeneic-free protocol for the generation of vmDA neurons from hPSCs for use in animal models of Parkinson’s disease. This protocol, utilizing Biolaminin 521 as a component, consistently increases both the yield and proportion of vmDA neural progenitors and neurons that display classical vmDA metabolic and electrophysiological properties. These findings may help in translation of hPSC-derived neurons into the clinic.
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